The basic setup is: radiation with a range of wavelengths is directed through a sample of interest, and a detector records which wavelengths were absorbed and to what extent the absorption occurred. We have been talking in general terms about how molecules absorb UV and visible light – now let's look at some actual examples of data from a UV-vis absorbance spectrophotometer. Therefore, by measuring the UV spectrum of a molecule, structural information can be derived about the nature of the conjugated pi electron system present. This energy gap depends on the conjugated system of the molecule being studied. If you recall from Section 3.3, the energy gap for π - π* transitions is smaller for conjugated systems than for isolated double bonds, and thus the wavelength absorbed is longer. The wavelength necessary to make the transition from π - π* in a conjugated molecule depends on the energy gap between the HOMO and LUMO. For more information on the HunterLab commitment to color technology and research, contact us today.\) We work together with leading scientists and researchers around the world to uncover new methods and uses for UV-VIS spectroscopy, and we are serious about making color measurement instrumentation that impacts our world. New applications for this analytical method are on the rise, and at HunterLab, we are excited to explore all the possibilities of UV-VIS spectroscopy. These light measurement tools are highly adaptable to meet specific needs and offer a durable method of analysis that is both portable and instantaneous. The benefits of spectrophotometers are especially applicable in the world of environmental science. These methods have been adapted for numerous areas of scientific research and have made strides in both biomedical and environmental engineering. Advances in this technology have provided scientists with a tool that offers a variety of applications in analytical research. UV-VIS spectrophotometry has developed in leaps and bounds over the past several decades. Precise quantification and data can alert environmental specialists to excess levels of these additives in our drinking water. UV-VIS spectroscopy can also be used to quantify levels of this element for both safety and effectiveness, and also provides an easy, non-destructive, chemical-free, and effective method of detection. Fluoride is another common additive to our drinking water. This identification is important because free residual chlorine is often considered a more effect disinfectant and can be used more effectively when properly detected in drinking water. UV-VIS spectroscopy offers an effective method for differentiating between the two. T wo main forms of residual chlorine exist, commonly referred to as ‘free chlorine’ or ‘combine chlorine’ residuals. A small portion of residual chlorine results from this process, so water must be accurately monitored to ensure that it is safe for human consumption. Those who maintain water sources often add chlorine due to its disinfecting purposes. Spectrophotometers for chlorine and fluoride quantification Spectrophotometers that measure light in both the ultraviolet and near-infrared regions are designed for portability as well as durability, making them well-suited to field work in environmental studies. Despite it being overlooked in the past, new developments in UV-VIS technology offer more information and data about water quality and safety. The EPA (Environmental Protection Agency) is continually searching for new ways to measure contaminants in drinking water. This process is immediate, portable, and cost-effective, making in a leading choice in analytical instrumentation for water safety analysis. However, new developments in UV-VIS spectroscopy now utilize the measurement of light absorption to quantify bacterial concentration levels in water samples accurately. Although standard bacterial testing is effective, it is also time-consuming and can take nearly two days for a complete analysis. One of the biggest concerns related to water contamination is bacteria. Spectrophotometric Analysis of Bacterial Water Contaminants
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